Activity-based ABL1 (Y245F/Y412F) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ABL1 (Y245F/Y412F)
Enzyme
Human ABL1 (Y245F/Y412F)
HGNC
ABL1 (Y245F/Y412F)
Synonym
Proto-oncogene tyrosine-protein kinase ABL1, c-ABL, p150, Abelson murine leukemia viral oncogene homolog 1
Scientific Information
P00519 (http://www.uniprot.org/uniprot/P00519)
Assay Properties
Substrates
Peptide substrate, [EAIYAAPFAKKK], 20 µM
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 59 |
| PP2 | 160 |
| AG1478 | 320 |
| EGFR/Erb Inhibitor | >100,000 |
| D-64406 | 190 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for ABL1 (Y245F/Y412F)
