Activity-based ABL1 Y253F kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
ABL1 Y253F
Enzyme
Human ABL1 Y253F, mutant
HGNC
ABL1 (Y253F)
Manning ID
ABL1
Synonym
ABL1, ABL, JTK7, p150, c-ABL, v-abl, ABL1 Y253F, ABL1 (Y253F)
UniProt
P00519 (ABL1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Poly(Ala,Glu,Lys,Tyr)6:2:5:1, ATP
Reaction
Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + 33P-ATP = 33P-Poly(Ala,Glu,Lys,Tyr)6:2:5:1 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 800 |
| Sorafenib | 5600 |
| Sunitinb | 8500 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | ABL1 Y253F |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 ABL1 Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – ABL1 Y253F (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0927-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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