Acetylcholinesterase (AChE) Biochemical Activity Assay for compound screening and profiling via absorbance measurements.
Target Overview
CAT#
AChE
Target
Acetylcholinesterase
Substrate
Acetylthicholine
Scientific Information
ACES (https://www.uniprot.org/uniprotkb/P22303/entry)
Assay Properties
Protocol
AChE + competing ligand incubation; add substrate + DTNB to initiate Ellman’s reaction; resultant thiocholine detected via absorbance measurement on Envision plate reader
Readout
Absorbance measurements at 412 nm (proportional to enzyme activity)
Reference Compound IC50
| Compound IC50 (nM) |
|---|
| Physostigmine (79.5) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound activity for AChE
PDF