Activity-based ACK1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
ACK1
Enzyme
Human ACK1, wt
HGNC
TNK2
Manning ID
ACK
Synonym
p21cdc42Hs, Activated CDC42 kinase 1, TNK2, ACK, ACK1
UniProt
Q07912 (ACK1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 4 |
| Sorafenib | 10000 |
| Sunitinb | 2400 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | ACK1 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – ACK1 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0493-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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