ACK1 Kinase Assay Service - Reaction Biology

Activity-based ACK1 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

ACK1

Enzyme

Human ACK1

HGNC

TNK2

Synonym

ACK, p21cdc42Hs, Activated CDC42 kinase 1

Substrates

Peptide substrate, [EAIYAAPFAKKK], 20 µM
ATP, 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q07912 (http://www.uniprot.org/uniprot/Q07912)

Screening Location

Malvern, USA

Reference compound IC50 for ACK1/TNK2