Adrenergic α1A Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
ADRA1A Binding
GPCR Target
Human α1A
Source
Membrane preparation from HEK-293 cells stably transfected with human Adrenergic α1A receptor.
Scientific Information
ADA1A (https://www.uniprot.org/uniprotkb/P35348/entry)
Assay Properties
Protocol
Radioligand filter binding to α1A membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| Prazosin (25) | Prazosin (10) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for α1A
PDF