AKT1 (E17K) NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
AKT1 (E17K)
AKT1 (E17K)
PKB; RAC; PRKBA; RAc-ALPHA
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
HEK293 cells transiently expressing NanoLuc- AKT1 (E17K) Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-10 and then treated with reference compound GSK690693 for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Binding of compound to target kinase
| Compounds | IC50 (M) |
|---|---|
| GSK690693 | 4.541e-008 |
Malvern, USA
