Activity-based AKT1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
AKT1
Enzyme
Human AKT1
HGNC
AKT1
Synonym
PKB, RAC, PRKBA, RAc-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, Protein kinase Ba, C-AKT
Substrates
Peptide substrate, [KGSGSGRPRTSSFAEG], 20 µM
ATP, 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
P31749 (http://www.uniprot.org/uniprot/P31749)
Screening Location
Malvern, USA
