AKT2 Kinase Assay Service - Reaction Biology

Activity-based AKT2 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

AKT2

Enzyme

Human AKT2

HGNC

AKT2

Synonym

PRKBB, PKBBETA, RAc-BETA

Substrates

Peptide substrate, [KGSGSGRPRTSSFAEG], 20 µM
ATP, 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

P31751 (http://www.uniprot.org/uniprot/P31751)

Screening Location

Malvern, USA

Reference compound IC50 for AKT2