Activity-based AKT3 aa106-479 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
AKT3 aa106-479
Enzyme
Human AKT3 aa106-479, wt
HGNC
AKT3 (aa106-479)
Manning ID
AKT3
Synonym
PKBG, PRKBG, STK-2, RAC-gamma, RAC-PK-gamma, AKT3, AKT3 aa106-479
UniProt
Q9Y243 (AKT3_HUMAN)
Kinase Type
Protein kinase
Kinase Group
AGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-GSK3(14-27), ATP
Reaction
RBER-GSK3(14-27) + 33P-ATP = 33P-RBER-GSK3(14-27) + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 79 |
| Sorafenib | 10000 |
| Sunitinb | 10000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | AKT3 aa106-479 |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – AKT3 aa106-479 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0436-0000-2 |
| Web Shop | Reaction Biology Online Shop |
PDF