AKT3 (E17K) Kinase Assay Service

Activity-based AKT3 (E17K) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

AKT3 (E17K)

Enzyme

Human AKT3 (E17K)

HGNC

AKT3 (E17K)

Synonym

PKBG, PKB gamma, PRKBG, RAC-gamma, RAC-PK-gamma, STK-2

Substrates

Peptide substrate, [GRPRTSSFAEG], 20 µM
ATP, 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q9Y243 (http://www.uniprot.org/uniprot/Q9Y243)

Screening Location

Malvern, USA

Reference compound IC50 for AKT3 (E17K)