ALK-EML4 (Eex20Aex20) Kinase Assay Service

Activity-based ALK-EML4 (Eex20Aex20) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.

Target Overview
CAT#

ALK, EML4 (Eex20Aex20)

Enzyme

Human EML4 – ALK (Eex20Aex20) fusion

HGNC

ALK-EML4 (Eex20Aex20)

Synonym

EML4: Echinoderm microtubule-associated protein-like 4, EMAP-4
ALK: ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246

Scientific Information

EML4: Q9HC35 (http://www.uniprot.org/uniprot/Q9HC35)
ALK: Q9UM73 (http://www.uniprot.org/uniprot/Q9UM73)

Assay Properties
Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP, 10 µM

Reaction

Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 4.0
PP2 24,000
AG1478 1,700
EGFR/Erb Inhibitor >100,000
D-64406 25,000
Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound IC50 for ALK-EML4 (Eex20Aex20)