ALK5/TGFBR1 Kinase Assay Service

Activity-based ALK5/TGFBR1 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

ALK5

Enzyme

Human ALK5

HGNC

TGFBR1

Synonym

ALK5, ACVRLK4

Substrates

Casein, 1 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

P36897 (http://www.uniprot.org/uniprot/P36897)

Screening Location

Malvern, USA

Reference compound IC50 for ALK5/TGFB-R1