ALK (F1174L)-NPM1 Kinase Assay Service

Activity-based ALK (F1174L)-NPM1 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

ALK (F1174L), NPM1-

Enzyme

Human NPM1-ALK (F1174L) fusion

HGNC

ALK (F1174L)-NPM1

Synonym

CD246, NBLST3

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

NPM1; P06748 (http://www.uniprot.org/uniprot/P06748)ALK; Q9UM73 (http://www.uniprot.org/uniprot/Q9UM73)

Screening Location

Malvern, USA

Reference compound IC50 for ALK (F1174L)-NPM1