Activity-based ALK (I1171N/D1203N) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ALK (I1171N/D1203N)
Enzyme
Human ALK (I1171N/D1203N)
HGNC
ALK (I1171N/D1203N)
Synonym
ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246
Scientific Information
Q9UM73 (http://www.uniprot.org/uniprot/Q9UM73)
Assay Properties
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP, 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 36 |
| PP2 | 33,700 |
| AG1478 | >100,000 |
| EGFR/ErbB Inhibitor | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for ALK (I1171N/D1203N)
