Activity-based ALK (L1196M/G1202R) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ALK (L1196M/G1202R)
Enzyme
Human ALK (L1196M/G1202R)
HGNC
ALK (L1196M/G1202R)
Synonym
ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246
Scientific Information
Q9UM73 (http://www.uniprot.org/uniprot/Q9UM73)
Assay Properties
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.0 |
| PP2 | 4,200 |
| AG1478 | 6,000 |
| EGFR/ErbB Inhibitor | >100,000 |
| D-64406 | 33,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinase Assays
Reference compound IC50 for ALK (L1196M/G1202R)
