α2A Adrenergic Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
α2A Binding
GPCR Target
Human α2A
Source
Membrane preparation from HEK-293 cells stably transfected with human α2A receptor.
Scientific Information
ADA2A (https://www.uniprot.org/uniprotkb/P08913/entry)
Assay Properties
Protocol
Radioligand filter binding to α2A membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| RX821002 (5.2) | RX821002 (5) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for α2A
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