AMPK (A1/B2/G1) Kinase Assay Service

Activity-based AMPK (A1/B2/G1) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

AMPK (A1/B2/G1)

Enzyme

Human AMPK (combination of A1/B2/G1 subunits)

HGNC

PRKAA1/PRKAB2/PRKAG1

Synonym

AMPK (A1/B2/G1)

Substrates

Peptide substrate, [HMRSAMSGLHLVKRR], 20 µM
AMP, 125 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
GW 5074	3,260
H-89	1,200
Ro 31-8220	95
Rottlerin	79,000
Staurosporine	<0.1

Scientific Information

Subunit A1: Q13131 (http://www.uniprot.org/uniprot/P49760)Subunit B2: O43741 (http://www.uniprot.org/uniprot/O43741)Subunit G1: P54619 (http://www.uniprot.org/uniprot/P54619)

Screening Location

Malvern, USA

Reference compound IC50 for AMPK (A1/B2/G1)