AMPK (A1/B2/G1) Kinase Assay Service

Activity-based AMPK (A1/B2/G1) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
AMPK (A1/B2/G1)
Enzyme
Human AMPK (combination of A1/B2/G1 subunits)
HGNC
PRKAA1/PRKAB2/PRKAG1
Synonym
AMPK (A1/B2/G1)
Substrates
Peptide substrate, [HMRSAMSGLHLVKRR], 20 µM
AMP, 125 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
Subunit A1: Q13131 (http://www.uniprot.org/uniprot/P49760)Subunit B2: O43741 (http://www.uniprot.org/uniprot/O43741)Subunit G1: P54619 (http://www.uniprot.org/uniprot/P54619)
Screening Location
Malvern, USA
Reference compound IC50 for AMPK (A1/B2/G1)