AMPK (A2/B1/G2) Kinase Assay Service

Activity-based AMPK (A2/B1/G2) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

AMPK (A2/B1/G2)

Enzyme

Human AMPK (combination of A2/B1/G2 subunits)

HGNC

PRKAA2/PRKAB1/PRKAG2

Synonym

AMPK (A2/B1/G2)

Substrates

Peptide substrate, [HMRSAMSGLHLVKRR], 20 µM
AMP, 125 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
GW 5074	1,000
H-89	310
Ro 31-8220	130
Rottlerin	>100,000
Staurosporine	0.2

Scientific Information

Subunit A2: P54646 (http://www.uniprot.org/uniprot/P54646)Subunit B1: Q9Y478 (http://www.uniprot.org/uniprot/Q9Y478)Subunit G2: Q9UGJ0 (http://www.uniprot.org/uniprot/Q9UGJ0)

Screening Location

Malvern, USA

Reference compound IC50 for AMPK (A2/B1/G2)