AMPK (A2/B2/G2) Kinase Assay Service

Activity-based AMPK (A2/B2/G2) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

AMPK (A2/B2/G2)

Enzyme

Human AMPK (combination of A2/B2/G2 subunits)

HGNC

PRKAA2/PRKAB2/PRKAG2

Synonym

AMPK (A2/B2/G2)

Substrates

Peptide substrate, [HMRSAMSGLHLVKRR], 20 µM
AMP, 125 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
GW 5074	2,800
H-89	990
Ro 31-8220	100
Staurosporine	<0.1

Scientific Information

Subunit A2: P54646 (http://www.uniprot.org/uniprot/P54646)Subunit B2: O43741 (http://www.uniprot.org/uniprot/O43741)Subunit G2: Q9UGJ0 (http://www.uniprot.org/uniprot/Q9UGJ0)

Screening Location

Malvern, USA

Reference compound IC50 for AMPK (A2/B2/G2)