Activity-based AMPKalpha1 aa1-312 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
AMPKalpha1 aa1-312
Enzyme
Human AMPKalpha1 aa1-312, wt
HGNC
PRKAA1 (aa1-312)
Manning ID
AMPKa1
Synonym
MGC33776, MGC57364, PRKAA1, AMPK-alpha1 aa1-312, AMPKalpha1 aa1-312, AMPKalpha1
UniProt
Q13131 (AAPK1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CAMK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 14 |
| Sorafenib | 2700 |
| Sunitinb | 52 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | AMPKalpha1 aa1-312 |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – AMPKalpha1 aa1-312 |
| Product Number |
0383-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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