AR (Androgen Nuclear Receptor) Functional Cell Reporter Antagonist Assay for compound screening and profiling via luminescence recordings.
Target Overview
CAT#
AR Functional Antagonist
GPCR Target
Human AR
Cell Line
Proprietary human cells engineered to constitutively express AR
Scientific Information
ANDR (https://www.uniprot.org/uniprotkb/P10275/entry)
Assay Properties
Protocol
Cells expressing AR incubated with antagonist + agonist (100 pM) for 24 h; luminescence measurement 30 min post application of detection reagent
Readout
Luminescence tracking changes in NFAT activity on Envision plate reader
Reference Compound IC50
| Compound IC50 (nM) | Agonist (nM) |
|---|---|
| Apalutamide (0.77) | 5αDH-11-kT (0.1) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound inhibition of AR
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