AR (Androgen Nuclear Receptor) Functional Cell Reporter Agonist Assay for compound screening and profiling via luminescence recordings.
Target Overview
CAT#
AR Functional Agonist
GPCR Target
Human AR
Cell Line
Proprietary human cells engineered to constitutively express AR
Scientific Information
ANDR (https://www.uniprot.org/uniprotkb/P10275/entry)
Assay Properties
Protocol
Cells expressing AR incubated with agonist for 24 h; luminescence measurement 30 min post application of detection reagent
Readout
Luminescence tracking changes in NFAT activity on Envision plate reader
Reference Compound EC50
| Compound EC50 (nM) |
|---|
| 5αDH-11-kT (0.028) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound activation of AR
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