Activity-based ARAF kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
ARAF
Enzyme
Human ARAF
HGNC
ARAF
Synonym
A-RA, ARAF1, PKS2, RAFA1
Substrates
MEK1 (K97R), 5 µM ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
Scientific Information
P10398 (http://www.uniprot.org/uniprot/P10398)
Screening Location
Malvern, USA
Reference compound IC50 for ARAF
