Activity-based ARAF YDYD kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
ARAF YDYD
Enzyme
Human ARAF YDYD, wt
HGNC
ARAF (Y301D/Y302D)
Manning ID
ARAF
Synonym
A-RA, ARAF1, PKS2, RAFA1, ARAF, ARAF YDYD
UniProt
P10398 (ARAF_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TKL
Assay Properties
Assay Format
33PanQinaseTM
Substrates
MEK1-K97M, ATP
Reaction
MEK1-K97M + 33P-ATP = 33P-MEK1-K97M + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Sorafenib | 73 |
| Sunitinib | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | ARAF YDYD |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – ARAF YDYD (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1768-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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