Activity-based ASH1L Methyltransferase Assay for compound screening and profiling via radiometric HotSpotTM methyltranferase assay.
Target Overview
CAT#
ASH1L
Enzyme
Human ASH1L
Synonym
Histone-lysine N-methyltransferase ASH1L, ASH1-like protein, Lysine N-methyltransferase 2H, Absent small and homeotic disks protein 1 homolog, KMT2H, KIAA1420
Substrates
Oligoucleosomes, 0.05 mg/ml
SAM, 1 µM
Assay Properties
Reaction
S-adenosyl-L-[methyl-³H]methionine + histone L- lysine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| SAH | 70,000 |
| Chaetocin | 52 |
| Sinefungin | 9,100 |
| Suramin | 390 |
Scientific Information
Q9NR48 (http://www.uniprot.org/uniprot/O9NR48)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Methyltransferase Assays.
Reference compound IC50 for ASH1L
PDF