Activity-based ASK1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
ASK1
Enzyme
Human ASK1, wt
HGNC
MAP3K5
Manning ID
MAP3K5
Synonym
ASK1, MAPKKK5, MEKK5, MAP3K5, ASK1/MAP3K5
UniProt
Q99683 (M3K5_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-GSK3(14-27), ATP
Reaction
RBER-GSK3(14-27) + 33P-ATP = 33P-RBER-GSK3(14-27) + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 38 |
| Sorafenib | 10000 |
| Sunitinib | 20000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | ASK1 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
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