ASK1/MAP3K5 Kinase Assay Service

Activity-based ASK1/MAP3K5 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

ASK1/MAP3K5

Enzyme

Human ASK1/MAP3K5

HGNC

MAP3K5

Synonym

ASK1, MAPKKK5, MEKK5

Substrates

MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q99683 (http://www.uniprot.org/uniprot/Q99683)

Screening Location

Malvern, USA

Reference compound IC50 for ASK1/MAP3K5