Ataxin-3 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
Ataxin-3/ATXN3
Enzyme
Human Ataxin-3/ATXN3
Synonym
Machado-Joseph disease protein 1, Spinocerebellar ataxia type 3 protein,ATX3, MJD, MJD1, SCA3
Substrate
Fluorogenic substrate [Ubiquitin-AMC], 100 nM
Assay Properties
Reaction
Cleavage of Ubiquitin for release of fluorescent AMC
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCHL1 I 2 | 153 |
| UCHL3 I | 298 |
Scientific Information
P54252 (http://www.uniprot.org/uniprot/P54252)
Screening Location
Malvern, USA
Reference Compound IC50 for Ataxin-3/ATXN3
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