ATM Kinase Assay Service - Reaction Biology

Activity-based ATM kinase assay for compound screening and profiling using HTRF kinase assay.

Target Overview

CAT#

ATM

Enzyme

Human ATM

HGNC

ATM

Synonym

TEL1, TELO1

Scientific Information

Q13315 (http://www.uniprot.org/uniprot/Q13315)

Assay Properties

Substrates

GST-p53, 100 nM
ATP 10 µM (Km), 1 mM

Reaction

Substrate + ATP = P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (10 µM ATP/Km)	IC50 (1 mM ATP)
AZD0156	0.06 nM	0.08 nM
KU-55933	1.5 nM	49 nM

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound inhibition of ATM