ATR Kinase Assay Service - Reaction Biology

Activity-based ATR kinase assay for compound screening and profiling using HTRF kinase assay.

Target Overview

CAT#

ATR

Enzyme

Human ATR

HGNC

ATR

Synonym

FRP1, Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1

Scientific Information

Q13535 (http://www.uniprot.org/uniprot/Q13535)

Assay Properties

Substrates

GST-p53, 75 nM
ATP Km (50 nM ATP), 10 µM, 1 mM

Reaction

Substrate + ATP = P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (Km ATP)	IC50 (10 µM ATP)	IC50 (1 mM ATP)
Berzosertib (VE-822)	0.2 nM	7.6 nM	4.2 µM
Elimusertib (BAY-1895344)	3.7 nM	0.6 µM	>100 µM
Camonsertib (RP-3500)	0.2 nM	3.1 nM	>100 µM
Tuvusertib	24 nM	4.7 µM	>100 µM

Screening Location

Malvern, PA, USA

Further Information

More information can be found on our website Kinase Assays

Reference compound inhibition of ATR

Reference compound IC50 Km ATP

Reference compound IC50 10 µM ATP

Reference compound IC50 1 mM ATP