Aurora A Kinase Assay Service

Activity-based Aurora A kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

Aurora A

Enzyme

Human Aurora A

HGNC

AURKA

Synonym

AurA, AIK, ARK1, AURA, BTAK, STK15

Substrates

Peptide substrate, [H-LRRASLG], 100 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

O14965 (http://www.uniprot.org/uniprot/O14965)

Screening Location

Malvern, USA

Reference compound IC50 for Aurora A