Aurora B (G160L) Kinase Assay Service

Activity-based Aurora B (G160L) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

Aurora B (G160L)

Enzyme

Human Aurora B (G160L)

HGNC

AURKB (G160L)

Synonym

AurB, AIK2, AIM1, ARK2, AurB, IPL1, STK5,

Substrates

Peptide substrate, [LRRASLG], 100 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q96GD4 (http://www.uniprot.org/uniprot/Q96GD4)

Screening Location

Malvern, USA

Reference compound IC50 for Aurora B (G160L)