Activity-based AuroraA kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
AuroraA
Enzyme
Human AuroraA, wt
HGNC
AURKA
Manning ID
AurA
Synonym
AIK, ARK1, AURA, BTAK, STK15, MGC34538, STK6, AURKA, Aurora-A, AurA, AuroraA, Aurora A
UniProt
O14965 (AURKA_HUMAN)
Kinase Type
Protein kinase
Kinase Group
Other
Assay Properties
Assay Format
33PanQinaseTM
Substrates
bio-4xCHKT, ATP
Reaction
bio-4xCHKT + 33P-ATP = 33P-bio-4xCHKT + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 17 |
| Sorafenib | 4200 |
| Sunitinib | 7600 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | AuroraA |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – AuroraA (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0166-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF