BAP1 deubiquitinase assay for compound screening and profiling via fluorescence-based quantification of enzyme activity.
Target Overview
CAT#
BAP1
Enzyme
Human BAP1
Synonym
Ubiquitin carboxyl-terminal hydrolase BAP1, BRCA1-associated protein 1,Cerebral protein 6, KIAA0272, hucep-6)
Substrate
Fluorogenic substrate [Ubiquitin-AMC], 100 nM
Assay Properties
Reaction
Cleavage of Ubiquitin for release of fluorescent AMC
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| UCHL1 I | 180 |
| UIPase I | >300 |
| UI1 | >300 |
Scientific Information
Q92560 (http://www.uniprot.org/uniprot/Q92560)
Screening Location
Malvern, USA
Reference Compound IC50 for BAP1
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