BAZ2A-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-BAZ2A-GST
Protein
Human BAZ2A bromodomain, GST-tagged
Synonyms
hWALp3; TTF-I-interacting protein 5; Tip5
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.1 mg/ml (2.5 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
37,5
Reference Compound ΔTms:
GSK2801 – 5.5 (red)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, SGC-CBP30, BET151, RVX-208 and PFI3)
Scientific Information
Q9UIF9 (http://www.uniprot.org/uniprot/Q9UIF9)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
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