BMX/ETK Kinase Assay Service - Reaction Biology

Activity-based BMX/ETK kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

BMX

Enzyme

Human BMX

HGNC

BMX

Synonym

ETK, PSCTK2, PSCTK3

Substrates

Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

P51813 (http://www.uniprot.org/uniprot/P51813)

Screening Location

Malvern, USA

Reference compound IC50 for BMX/ETK