BPTF Bromodomain Assay Service (AlphaScreen)

BPTF-[BRD]-His Reader Domain Assay for compound screening and profiling using the AlphaScreen assay format to measure compound binding to the target.

Target Overview

CAT#

BPTF-[BRD]-His

Enzyme

Human BPTF-[BRD]-His

Synonyms

Nucleosome-remodeling factor subunit BPTF, Bromodomain and PHD finger-containing transcription factor, Fetal Alz-50 clone 1 protein, Fetal Alzheimer antigen, FAC1, FALZ

Substrates

Histone H4 peptide (1-21) K5/8/12/16Ac

Assay Properties

Reaction

AlphaScreen Binding assay

Readout

Fluorescence

Reference Compound IC50s

Compounds	IC50 (nM)
JQ-1	15,000
Bromosporine	5,500
SGC-CBP30	15,000

Scientific Information

Q12830 (http://www.uniprot.org/uniprot/Q12830)

Screening Location

Malvern, USA

Further information

More information can be found on our website Reader Domain Assay Services.

Reference Compound IC50 for BPTF-[BRD]