BRAF-FAM131B Kinase Assay Service

Activity-based BRAF-FAM131B kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

BRAF, FAM131B-

Enzyme

Human FAM131B-BRAF fusion

HGNC

BRAF-FAM131B

Synonym

B-Raf, B-raf-1, BRAF1, RAFB1

Substrates

MEK1 (K97R), 5 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

FAM131B: Q86XD5 (http://www.uniprot.org/uniprot/Q86XD5)BRAF: P15056 (http://www.uniprot.org/uniprot/P15056)

Screening Location

Malvern, USA

Reference compound IC50 for BRAF-FAM131B