BRAF-SRGAP3 (Sex12Bex9) Kinase Assay Service

Activity-based BRAF-SRGAP3 (Sex12Bex9) kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

BRAF, SRGAP3-

Enzyme

Human SRGAP3-BRAF fusion

HGNC

BRAF

Synonym

B-Raf, B-raf-1, BRAF1, RAFB1

Substrates

MEK1 (K97R), 5 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

SRGAP3: O43295 (http://www.uniprot.org/uniprot/O43925)BRAF: P15056 (http://www.uniprot.org/uniprot/P15056)

Screening Location

Malvern, USA

Reference compound IC50 for BRAF-SRGAP3 (Sex12Bex9)