BRAF Kinase Assay Service - Reaction Biology

Activity-based BRAF kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

BRAF

Enzyme

Human BRAF

HGNC

BRAF

Synonym

B-Raf, B-raf-1, BRAF1, RAFB1

Substrates

MEK1 (K97R), 3 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Compounds	IC50 (nM)
GW 5074	24

Scientific Information

P15056 (http://www.uniprot.org/uniprot/P15056)

Screening Location

Malvern, USA

Reference compound IC50 for BRAF