BRD1-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-BRD1-H
Protein
Human BRD1 bromodomain, His-tagged
Synonyms
BR140-like protein, BRL; Bromodomain and PHD finger-containing protein 2, BRPF-2
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.125 mg/ml (6.9 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
46
Reference Compound ΔTms:
Bromosporine – 3 (Blue)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, SGC-CBP30, BET151, RVX-208, GSK2801 and PFI3)
Scientific Information
O95696 (http://www.uniprot.org/uniprot/O95696)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
