Surface Plasmon Resonance (SPR) technique for evaluating direct binding of BRD2-2 to molecular inhibitors
Target Overview
Target
Bromodomain-containing protein 2 domain 2 (aa 344-454)
HGNC symbol
BRD2
Synonyms
O27.1.1, Really interesting new gene 3 protein
Uniprot page
P25440 (https://www.uniprot.org/uniprot/P25440)
Protein Source:
Reaction Biology Cat# RD-11-154
Screening location
Malvern, PA, USA
Further information
More information can be found on our website SPR Assay Services for Drug Discovery
Binding of three BET bromodomain inhibitors to BRD2-2
The data was collected on a Biacore 8K using multi-cycle kinetics. The data was analyzed using a 1:1 kinetic binding model or steady state affinity model to determine binding affinity (KD).
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