BRD4-1-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-BRD4-1-GST
Protein
Human BRD4-1 bromodomain, GST-tagged
Synonyms
HUNK1
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.075 mg/ml (1.8 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
43,5
Reference Compound ΔTms:
JQ1 – 6.5 (Orange)PFI1 – 5 (Pink)BET151 – 6.5 (Blue)No stabilization was observed in the presence of other control compounds (CBP112, RVX-208, GSK2801 and PFI3)
Scientific Information
O60885 (http://www.uniprot.org/uniprot/O60885)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
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