BRD9-His Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-BRD9-H
Protein
Human BRD9 bromodomain, His-tagged
Synonyms
Rhabdomyosarcoma antigen MU-RMS-40.8)
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.1 mg/ml (6.3 µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
45,5
Reference Compound ΔTms:
Bromosporine – 8 (Blue)No stabilization was observed in the presence of other control compounds (JQ1, PFI1, CBP112, SGC-CBP30, BET151, GSK2801 and PFI3)
Scientific Information
Scientific Information: Q9H8M2 (http://www.uniprot.org/uniprot/Q9H8M2)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
