Surface Plasmon Resonance (SPR) technique for evaluating direct binding of BRDT-1 to molecular inhibitors
Target Overview
Target
Bromodomain testis-specific protein domain 1 (aa 21-137)
HGNC symbol
BRDT
Synonyms
Cancer/testis antigen 9, RING3-like protein
Uniprot page
Q58F21 (https://www.uniprot.org/uniprot/Q58F21)
Protein Source:
Reaction Biology Cat# RD-11-169
Screening location
Malvern, PA, USA
Further information
More information can be found on our website SPR Assay Services for Drug Discovery
Binding of three BET bromodomain inhibitors to BRDT-1
The data was collected on a Biacore 8K using multi-cycle kinetics. The data was analyzed using a 1:1 kinetic binding model or steady state affinity model to determine binding affinity (KD).
PDF