BRK Kinase Activity Assay Service

Activity-based BRK kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview
RBE Target ID

BRK

Enzyme

Human BRK, wt

HGNC

PTK6

Manning ID

BRK

Synonym

PTK6, BRK

UniProt

Q13882 (PTK6_HUMAN)

Kinase Type

Protein kinase

Kinase Group

TK

Assay Properties
Assay Format

33PanQinaseTM

Substrates

PolyEY, ATP

Reaction

PolyEY + 33P-ATP = 33P-PolyEY + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 1543
Sorafenib 10000
Sunitinib 6100
Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability
Screening Opportunities
FreeChoice BRK
355 wt Profiler Yes
355 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein Yes; ProQinaseTM – BRK
(Activity verified by 33PanQinaseTM-Assay)
Product Number 0181-0000-1
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