BRPF1b-GST Reader Domain Assay for compound screening and profiling using the thermoshift assay format to measure compound binding to the target.
Target Overview
CAT#
Tm-BRPF1b-GST
Protein
Human BRPF1b bromodomain, GST-tagged
Synonyms
Peregrin; Bromodomain and PHD finger-containing protein 1; BR140
Assay Properties
Readout
Thermal stability change after ligand binding
Concentration of protein in assay
0.1 mg/ml (2.5µM)
Concentration of compound in assay
25 µM
Apo form Tm (°C)
42
Reference Compound ΔTms:
Bromosporine – 4.5 (Blue)PFI1 – 2 (Pink)RVX – 208 – 1.5 (Purple)No stabilization was observed in the presence of other control compounds (JQ1, CBP112, SGC-CBP30, BET151, GSK2801 and PFI3)
Scientific Information
P55201 (http://www.uniprot.org/uniprot/P55201)
Screening Location
Malvern, USA
Further information
More information can be found on our website Reader Domain Assay Services.
Melt Curve
Melt Peak
PDF