BRSK1 Kinase Assay Service - Reaction Biology

Activity-based BRSK1 kinase assay for compound screening and profiling via radiometric HotSpot^TM kinase assay.

Target Overview

CAT#

BRSK1

Enzyme

Human BRSK1

HGNC

BRSK1

Synonym

KIAA1811

Substrates

Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM

Assay Properties

Reaction

Substrate + [γ-³³P]-ATP = ³³P-Substrate + ADP

Readout

Enzymatic activity

Reference Compound IC50s

Scientific Information

Q8TDC3 (http://www.uniprot.org/uniprot/Q8TDC3)

Screening Location

Malvern, USA

Reference compound IC50 for BRSK1