Activity-based BRSK2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
BRSK2
Enzyme
Human BRSK2, wt
HGNC
BRSK2
Manning ID
BRSK2
Synonym
C11orf7, PEN11B, SAD1, STK29, FLJ41362, HUSSY-12, BRSK2
UniProt
Q8IWQ3 (BRSK2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CAMK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1.3 |
| Sorafenib | 10000 |
| Sunitinib | 3300 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | BRSK2 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – BRSK2 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1524-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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